Coding

Part:BBa_K4716998

Designed by: LIAO WENYU   Group: iGEM23_UM-Macau   (2023-10-10)


NorR (PnorV regulator)

NorR has two very important functions, one is that it can bind to the promoter PnorV (BBa_K4716997) on DNA, and the other is that it can also bind more preferentially to NO in the presence of nitric oxide in the environment. NorR binds to DNA and inhibits transcription initiation; When NO molecules are present in the environment，NorR exposes its AAA+ ATPase domain, nonbinding to DNA molecule (PnorV promoter) will release its inhibition of downstream gene expression, allowing genes downstream of the PnorV promoter to be transcribed and expressed. In our project, NorR will be mediated by the ProA promoter (BBa_K4716999) to repress proline endopeptidase (BBa_K4716996 and BBa_K4716995) expression downstream of the PnorV promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 856
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1080


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Categories
Parameters
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